CC₅₀/IC₅₀/EC₅₀: 0.631 mM
Dispersion: 0.047 (ND)
Exposure: 36 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Gibco)

CC₅₀/IC₅₀/EC₅₀: 20 mM
Exposure: 24 h
Assay: CellQuanti-Blue
CellQuanti-Blue cell viability
The CellQuanti-Blue assay is a fluorometric method employed to evaluate cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells, generating a fluorescent signal proportional to the number of viable cells.

L929
(NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; ,NCTC clone 929; L929(NCTC); Clone 929; Earles's cells; Earle's cell)
ATCC: ccl-1
Cellosaurus: CVCL_0462
 
Species: Mus musculus (mouse)
Tissue: Adipose, Areolar, Subcutaneous connective tissue
Morphology: Fibroblast
 
Medium: DMEM (PAN-Biotech)

CC₅₀/IC₅₀/EC₅₀: 5.4 mM
Exposure: 24 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

CaCo-2
(Caco2; Caco-2/ATCC; Caco-II)
ATCC: htb-37
Cellosaurus: CVCL_0025
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Colorectal adenocarcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial-like
 
Medium: MEM (Biochrom AG)

CC₅₀/IC₅₀/EC₅₀: 9.91 mM
Exposure: 24 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Biochrom AG)

CC₅₀/IC₅₀/EC₅₀: 0.875 mM
Dispersion: 0.037 (ND)
Exposure: 36 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Gibco)

CC₅₀/IC₅₀/EC₅₀: 1.304 mM
Dispersion: 0.13 (ND)
Exposure: 36 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Gibco)

CC₅₀/IC₅₀/EC₅₀: 20 mM
Exposure: 24 h
Assay: CellQuanti-Blue
CellQuanti-Blue cell viability
The CellQuanti-Blue assay is a fluorometric method employed to evaluate cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells, generating a fluorescent signal proportional to the number of viable cells.

L929
(NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; ,NCTC clone 929; L929(NCTC); Clone 929; Earles's cells; Earle's cell)
ATCC: ccl-1
Cellosaurus: CVCL_0462
 
Species: Mus musculus (mouse)
Tissue: Adipose, Areolar, Subcutaneous connective tissue
Morphology: Fibroblast
 
Medium: DMEM (PAN-Biotech)

CC₅₀/IC₅₀/EC₅₀: 2 mM
Exposure: 72 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: >20 mM
Exposure: 72 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: 0.88 mM
Dispersion: 0.16 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.88 mM
Dispersion: 0.31 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.88 mM
Dispersion: 0.32 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.18 mM
Dispersion: 0.06 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.18 mM
Dispersion: 0.15 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.18 mM
Dispersion: 0.19 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.24 mM
Dispersion: 0.12 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.087 mM
Dispersion: 0.09 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.47 mM
Dispersion: 0.39 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.87 mM
Dispersion: 0.36 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.83 mM
Dispersion: 0.56 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.12 mM
Dispersion: 0.25 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 5 mM
Dispersion: 2.36 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 2.51 mM
Dispersion: 0.66 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 3.12 mM
Dispersion: 1.26 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.81 mM
Dispersion: 0.89 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.71 mM
Dispersion: 0.75 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.09 mM
Dispersion: 0.71 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.7 mM
Dispersion: 0.5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: 0.06613 mM
Dispersion: 0.01606 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.3721 mM
Dispersion: 0.06792 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 2.5 mM
Dispersion: 0.5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: 0.1423 mM
Dispersion: 0.00032 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.1938 mM
Dispersion: 0.05504 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: ~0.4 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: 0.09197 mM
Dispersion: 0.00802 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.1956 mM
Dispersion: 0.00336 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: <0.4 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: <0.5 mM
Exposure: 48 h
Assay: WST-8
Water-Soluble Tetrazolium-8
The WST-8 assay is a colorimetric method similar to WST-1 assay, utilizing a tetrazolium salt that is cleaved by cellular enzymes to form a soluble formazan dye, enabling quantification of cell viability and proliferation.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.05879 mM
Dispersion: 0.00452 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.06678 mM
Dispersion: 0.01042 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: <0.5 mM
Exposure: 48 h
Assay: WST-8
Water-Soluble Tetrazolium-8
The WST-8 assay is a colorimetric method similar to WST-1 assay, utilizing a tetrazolium salt that is cleaved by cellular enzymes to form a soluble formazan dye, enabling quantification of cell viability and proliferation.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 35 mM
Exposure: 24 h
Assay: CellQuanti-Blue
CellQuanti-Blue cell viability
The CellQuanti-Blue assay is a fluorometric method employed to evaluate cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells, generating a fluorescent signal proportional to the number of viable cells.

L929
(NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; ,NCTC clone 929; L929(NCTC); Clone 929; Earles's cells; Earle's cell)
ATCC: ccl-1
Cellosaurus: CVCL_0462
 
Species: Mus musculus (mouse)
Tissue: Adipose, Areolar, Subcutaneous connective tissue
Morphology: Fibroblast
 
Medium: DMEM (PAN-Biotech)

CC₅₀/IC₅₀/EC₅₀: 0.0235 mM
Exposure: 72 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.0172 mM
Exposure: 72 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.00891 mM
Dispersion: 0.00228 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.04823 mM
Dispersion: 0.00468 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.04124 mM
Dispersion: 0.0033 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.08854 mM
Dispersion: 0.02112 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.9 mM
Dispersion: 0.5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: 0.02598 mM
Dispersion: 0.00696 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.1784 mM
Dispersion: 0.10658 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.02911 mM
Dispersion: 0.0044 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.05448 mM
Dispersion: 0.00884 (95% CI)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.5 mM
Exposure: 48 h
Assay: WST-8
Water-Soluble Tetrazolium-8
The WST-8 assay is a colorimetric method similar to WST-1 assay, utilizing a tetrazolium salt that is cleaved by cellular enzymes to form a soluble formazan dye, enabling quantification of cell viability and proliferation.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.3 mM
Dispersion: 0.5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: 2.4 mM
Dispersion: 0.5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: <0.4 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: <0.4 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HaCat
(HaCaT; HaCAT; HACAT; Hacat)
Cellosaurus: CVCL_0038
 
Species: Homo sapiens (human)
Cell type: Keratinocyte
Tissue: Epidermis, Skin
Morphology: Keratinocyte
 
Medium: DMEM (Life technologies)

CC₅₀/IC₅₀/EC₅₀: 5.35 mM
Dispersion: 3.69-7.01 (95% CI)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

3215 LS
 
Species: Homo sapiens (human)
Cell type: Fibroblast
 
Medium: DMEM/F-12 (Gibco)
Notes
As stated in the original publication (10.1021/acsmedchemlett.5b00258), courtesy of E. Kopantsev (M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry RAS).

CC₅₀/IC₅₀/EC₅₀: 22.4 mM
Dispersion: 3.14-41.67 (95% CI)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

3215 LS
 
Species: Homo sapiens (human)
Cell type: Fibroblast
 
Medium: DMEM/F-12 (Gibco)
Notes
As stated in the original publication (10.1021/acsmedchemlett.5b00258), courtesy of E. Kopantsev (M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry RAS).

CC₅₀/IC₅₀/EC₅₀: 9.97 mM
Dispersion: 4.26-15.69 (95% CI)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

3215 LS
 
Species: Homo sapiens (human)
Cell type: Fibroblast
 
Medium: DMEM/F-12 (Gibco)
Notes
As stated in the original publication (10.1021/acsmedchemlett.5b00258), courtesy of E. Kopantsev (M.M. Shemyakin and Yu.A. Ovchinnikov Institute of Bioorganic Chemistry RAS).

CC₅₀/IC₅₀/EC₅₀: 0.02562 mM
Dispersion: 0.0002 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

A375
(A 375; A-375; A375-MEL; A375-mel; A375mel)
ATCC: crl-1619
Cellosaurus: CVCL_0132
 
Species: Homo sapiens (human)
Disease: Malignant melanoma
Tissue: Skin
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: >0.05 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

BGC-823
(BGC823)
Cellosaurus: CVCL_3360
 
Species: Homo sapiens (human)
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial-like
 
Medium: RPMI 1640
Notes
Problematic cell line; contaminated; shown to be a HeLa derivative. Originally thought to be derived from a primary gastric adenocarcinoma.

CC₅₀/IC₅₀/EC₅₀: 0.02291 mM
Dispersion: 0.0018 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.02174 mM
Dispersion: 0.00101 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HELF
(Human embryonic lung fibroblast)
 
Species: Homo sapiens (human)
Cell type: Fibroblast
Tissue: Embryo, Lung
Morphology: Fibroblast
 
Medium: RPMI 1640
Notes
Derived from normal human embryonic lung tissue (as described in 10.1111/cbdd.12612).

CC₅₀/IC₅₀/EC₅₀: 0.00525 mM
Dispersion: 0.00036 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

A375
(A 375; A-375; A375-MEL; A375-mel; A375mel)
ATCC: crl-1619
Cellosaurus: CVCL_0132
 
Species: Homo sapiens (human)
Disease: Malignant melanoma
Tissue: Skin
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.00846 mM
Dispersion: 0.00109 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

BGC-823
(BGC823)
Cellosaurus: CVCL_3360
 
Species: Homo sapiens (human)
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial-like
 
Medium: RPMI 1640
Notes
Problematic cell line; contaminated; shown to be a HeLa derivative. Originally thought to be derived from a primary gastric adenocarcinoma.

CC₅₀/IC₅₀/EC₅₀: 0.00734 mM
Dispersion: 1.0E-5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.00935 mM
Dispersion: 0.00061 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HELF
(Human embryonic lung fibroblast)
 
Species: Homo sapiens (human)
Cell type: Fibroblast
Tissue: Embryo, Lung
Morphology: Fibroblast
 
Medium: RPMI 1640
Notes
Derived from normal human embryonic lung tissue (as described in 10.1111/cbdd.12612).

CC₅₀/IC₅₀/EC₅₀: 0.00362 mM
Dispersion: 0.00025 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

A375
(A 375; A-375; A375-MEL; A375-mel; A375mel)
ATCC: crl-1619
Cellosaurus: CVCL_0132
 
Species: Homo sapiens (human)
Disease: Malignant melanoma
Tissue: Skin
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.00592 mM
Dispersion: 0.00067 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

BGC-823
(BGC823)
Cellosaurus: CVCL_3360
 
Species: Homo sapiens (human)
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial-like
 
Medium: RPMI 1640
Notes
Problematic cell line; contaminated; shown to be a HeLa derivative. Originally thought to be derived from a primary gastric adenocarcinoma.

CC₅₀/IC₅₀/EC₅₀: 0.0069 mM
Dispersion: 0.00018 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.01161 mM
Dispersion: 0.00092 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HELF
(Human embryonic lung fibroblast)
 
Species: Homo sapiens (human)
Cell type: Fibroblast
Tissue: Embryo, Lung
Morphology: Fibroblast
 
Medium: RPMI 1640
Notes
Derived from normal human embryonic lung tissue (as described in 10.1111/cbdd.12612).

CC₅₀/IC₅₀/EC₅₀: 0.00175 mM
Dispersion: 0.00021 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals. 
Presumable mechanism
Presumable cytotoxicity mechanism:
Apoptosis, ROS elevation, loss of mitochondrial membrane potential, CASP3 and CASP9 activation, cytochrome c elevation; the compound was predominantly found in mitochondria

A375
(A 375; A-375; A375-MEL; A375-mel; A375mel)
ATCC: crl-1619
Cellosaurus: CVCL_0132
 
Species: Homo sapiens (human)
Disease: Malignant melanoma
Tissue: Skin
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.00379 mM
Dispersion: 0.00095 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals. 
Presumable mechanism
Presumable cytotoxicity mechanism:
ROS elevation, loss of mitochondrial membrane potential

BGC-823
(BGC823)
Cellosaurus: CVCL_3360
 
Species: Homo sapiens (human)
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial-like
 
Medium: RPMI 1640
Notes
Problematic cell line; contaminated; shown to be a HeLa derivative. Originally thought to be derived from a primary gastric adenocarcinoma.

CC₅₀/IC₅₀/EC₅₀: 0.00471 mM
Dispersion: 0.00076 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals. 
Presumable mechanism
Presumable cytotoxicity mechanism:
ROS elevation, loss of mitochondrial membrane potential

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.01217 mM
Dispersion: 8.0E-5 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HELF
(Human embryonic lung fibroblast)
 
Species: Homo sapiens (human)
Cell type: Fibroblast
Tissue: Embryo, Lung
Morphology: Fibroblast
 
Medium: RPMI 1640
Notes
Derived from normal human embryonic lung tissue (as described in 10.1111/cbdd.12612).

CC₅₀/IC₅₀/EC₅₀: 0.00298 mM
Dispersion: 0.00041 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

A375
(A 375; A-375; A375-MEL; A375-mel; A375mel)
ATCC: crl-1619
Cellosaurus: CVCL_0132
 
Species: Homo sapiens (human)
Disease: Malignant melanoma
Tissue: Skin
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.00502 mM
Dispersion: 0.0019 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

BGC-823
(BGC823)
Cellosaurus: CVCL_3360
 
Species: Homo sapiens (human)
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial-like
 
Medium: RPMI 1640
Notes
Problematic cell line; contaminated; shown to be a HeLa derivative. Originally thought to be derived from a primary gastric adenocarcinoma.

CC₅₀/IC₅₀/EC₅₀: 0.00857 mM
Dispersion: 0.00339 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HepG2
(HEP G2; HepG2; HEPG2)
ATCC: hb-8065
Cellosaurus: CVCL_0027
 
Species: Homo sapiens (human)
Disease: Hepatocellular carcinoma
Tissue: Liver
Morphology: Epithelial-like
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: 0.0086 mM
Dispersion: 0.00126 (ND)
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HELF
(Human embryonic lung fibroblast)
 
Species: Homo sapiens (human)
Cell type: Fibroblast
Tissue: Embryo, Lung
Morphology: Fibroblast
 
Medium: RPMI 1640
Notes
Derived from normal human embryonic lung tissue (as described in 10.1111/cbdd.12612).

CC₅₀/IC₅₀/EC₅₀: 1.99 mM
Dispersion: 1.72-2.37 (95% CI)
Exposure: 24 h
Assay: CellTiter-Blue
CellTiter-Blue cell viability
The CellTiter-Blue assay is a fluorometric assay used to assess cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells to produce a fluorescent signal, indicating cell viability.

4T1-Luc
 
Species: Mus musculus (mouse)
Disease: Breast cancer
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM
Notes
Derived from 4T1 mammary carcinoma, engineered to express luciferase for bioluminescent imaging.

CC₅₀/IC₅₀/EC₅₀: 28.6 mM
Dispersion: 25.5-32 (95% CI)
Exposure: 24 h
Assay: CellTiter-Blue
CellTiter-Blue cell viability
The CellTiter-Blue assay is a fluorometric assay used to assess cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells to produce a fluorescent signal, indicating cell viability.

4T1-Luc
 
Species: Mus musculus (mouse)
Disease: Breast cancer
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM
Notes
Derived from 4T1 mammary carcinoma, engineered to express luciferase for bioluminescent imaging.

CC₅₀/IC₅₀/EC₅₀: 3.28 mM
Dispersion: 2.43-4.96 (95% CI)
Exposure: 24 h
Assay: CellTiter-Blue
CellTiter-Blue cell viability
The CellTiter-Blue assay is a fluorometric assay used to assess cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells to produce a fluorescent signal, indicating cell viability.

4T1-Luc
 
Species: Mus musculus (mouse)
Disease: Breast cancer
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM
Notes
Derived from 4T1 mammary carcinoma, engineered to express luciferase for bioluminescent imaging.

CC₅₀/IC₅₀/EC₅₀: 10.7 mM
Dispersion: 9.14-12.7 (95% CI)
Exposure: 24 h
Assay: CellTiter-Blue
CellTiter-Blue cell viability
The CellTiter-Blue assay is a fluorometric assay used to assess cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells to produce a fluorescent signal, indicating cell viability.

4T1-Luc
 
Species: Mus musculus (mouse)
Disease: Breast cancer
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM
Notes
Derived from 4T1 mammary carcinoma, engineered to express luciferase for bioluminescent imaging.

CC₅₀/IC₅₀/EC₅₀: 4.8 mM
Dispersion: 3.75-6.52 (95% CI)
Exposure: 24 h
Assay: CellTiter-Blue
CellTiter-Blue cell viability
The CellTiter-Blue assay is a fluorometric assay used to assess cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells to produce a fluorescent signal, indicating cell viability.

4T1-Luc
 
Species: Mus musculus (mouse)
Disease: Breast cancer
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM
Notes
Derived from 4T1 mammary carcinoma, engineered to express luciferase for bioluminescent imaging.

CC₅₀/IC₅₀/EC₅₀: 1.12 mM
Dispersion: 0.971-1.31 (95% CI)
Exposure: 24 h
Assay: CellTiter-Blue
CellTiter-Blue cell viability
The CellTiter-Blue assay is a fluorometric assay used to assess cell viability by measuring the reduction of a dye (resazurin) by metabolically active cells to produce a fluorescent signal, indicating cell viability.

4T1-Luc
 
Species: Mus musculus (mouse)
Disease: Breast cancer
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM
Notes
Derived from 4T1 mammary carcinoma, engineered to express luciferase for bioluminescent imaging.

CC₅₀/IC₅₀/EC₅₀: 7.3 mM
Exposure: 72 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: >20 mM
Exposure: 72 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: 6.3 mM
Exposure: 72 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: 3.9 mM
Exposure: 72 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: 1.51 mM
Dispersion: 0.09 (SD)
Exposure: 72 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

NHDF
(Normal human dermal fibroblast cell line)
 
Species: Homo sapiens (human)
Tissue: Skin
 
Medium: DMEM (Gibco)
Notes
Derived from normal human dermal tissue.

CC₅₀/IC₅₀/EC₅₀: 8 mM
Dispersion: 0.85 (SE)
Exposure: 48 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM (Hyclone)

CC₅₀/IC₅₀/EC₅₀: 0.33 mM
Dispersion: 0.02 (SE)
Exposure: 48 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM (Hyclone)

CC₅₀/IC₅₀/EC₅₀: 6.41 mM
Dispersion: 0.71 (SE)
Exposure: 48 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM (Hyclone)

CC₅₀/IC₅₀/EC₅₀: 0.183 mM
Dispersion: 0.032 (SD)
Exposure: 72 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM (Gibco)

CC₅₀/IC₅₀/EC₅₀: 0.299 mM
Dispersion: 0.018 (SD)
Exposure: 72 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM (Gibco)

CC₅₀/IC₅₀/EC₅₀: 0.313 mM
Dispersion: 0.014 (SD)
Exposure: 72 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

U251
(U-251 MG; U-251-MG; U-251_MG; U251-MG; U251MG; U-251; U251; U251n; U251N; 251 MG; 251MG; 251 MG(6))
Cellosaurus: CVCL_0021
 
Species: Homo sapiens (human)
Disease: Astrocytoma
Tissue: Brain, Parietal lobe
 
Medium: DMEM (Gibco)

CC₅₀/IC₅₀/EC₅₀: 2.22 mM
Dispersion: 0.36 (SD)
Exposure: 72 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

NHDF
(Normal human dermal fibroblast cell line)
 
Species: Homo sapiens (human)
Tissue: Skin
 
Medium: DMEM (Gibco)
Notes
Derived from normal human dermal tissue.

CC₅₀/IC₅₀/EC₅₀: >2.52 mM
Exposure: unknown
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: >4.7322 mM
Exposure: unknown
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: 0.00578 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

C6
(C-6; C 6; RGC-6; RGC6; RGc6)
ATCC: ccl-107
Cellosaurus: CVCL_0194
 
Species: Rattus norvegicus (rat)
Cell type: Glial cell
Disease: Glioma
Tissue: Brain
Morphology: Fibroblast

CC₅₀/IC₅₀/EC₅₀: 0.04 mM
Exposure: 2 h
Assay: Hemoglobin release
Hemoglobin release assay
Hemoglobin release assay is a method used to assess cell membrane integrity or cytotoxicity by quantifying the release of hemoglobin from lysed cells into the surrounding medium.

RBC
(Human red blood cells)
 
Species: Homo sapiens (human)
Cell type: Red blood cell
Tissue: Blood
Morphology: Biconcave discs
Notes
Derived from human blood samples. Red blood cells are not typically considered cell lines in the same sense as immortalized or cultured cell lines. They are primary cells obtained directly from blood donations.

CC₅₀/IC₅₀/EC₅₀: 24.2 mM
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

L929
(NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; ,NCTC clone 929; L929(NCTC); Clone 929; Earles's cells; Earle's cell)
ATCC: ccl-1
Cellosaurus: CVCL_0462
 
Species: Mus musculus (mouse)
Tissue: Adipose, Areolar, Subcutaneous connective tissue
Morphology: Fibroblast
 
Medium: MEM (Corning)

CC₅₀/IC₅₀/EC₅₀: 26.3 mM
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

L929
(NCTC 929; NCTC-929; NCTC929; NCTC-929L; L cell; L cells; L-cell; L-cells; L cell line; L; Strain L-929; L-929; L 929; ,NCTC clone 929; L929(NCTC); Clone 929; Earles's cells; Earle's cell)
ATCC: ccl-1
Cellosaurus: CVCL_0462
 
Species: Mus musculus (mouse)
Tissue: Adipose, Areolar, Subcutaneous connective tissue
Morphology: Fibroblast
 
Medium: MEM (Corning)

CC₅₀/IC₅₀/EC₅₀: 9.8 mM
Dispersion: 0.7 (SD)
Exposure: 24 h
Assay: AlamarBlue
AlamarBlue cell viability
The AlamarBlue assay is a fluorometric method employed to assess cell viability and proliferation by measuring the reduction of resazurin dye by metabolically active cells, resulting in a fluorescent signal proportional to the number of viable cells.

HCE
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Tissue: Cornea
Morphology: Epithelial
 
Medium: DMEM/F-12 (Gibco)
Notes
Derived from primary cultures of human corneal epithelial cells. Possibly HCE-2 cell line (as described in 10.1002/chem.201704924).

CC₅₀/IC₅₀/EC₅₀: 1.2522 mM
Dispersion: 1.15-1.35 (95% CI)
Exposure: 48 h
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Invitrogen Life Technologies)

CC₅₀/IC₅₀/EC₅₀: >0.5 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: >0.5 mM
Exposure: 48 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: RPMI 1640

CC₅₀/IC₅₀/EC₅₀: >0.5 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

K562
(K-562; K.562; K 562; KO; GM05372; GM05372E)
Cellosaurus: CVCL_0004
 
Species: Homo sapiens (human)
Disease: Chronic myelogenous leukemia
Tissue: Bone marrow
Morphology: Lymphoblast

CC₅₀/IC₅₀/EC₅₀: >0.5 mM
Exposure: 48 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

K562
(K-562; K.562; K 562; KO; GM05372; GM05372E)
Cellosaurus: CVCL_0004
 
Species: Homo sapiens (human)
Disease: Chronic myelogenous leukemia
Tissue: Bone marrow
Morphology: Lymphoblast

CC₅₀/IC₅₀/EC₅₀: >0.5 mM
Exposure: 24 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HUVEC
(HUV-EC-C; Primary Umbilical Vein Endothelial Cells)
ATCC: pcs-100-010
 
Species: Homo sapiens (human)
Cell type: Endothelial cell
Tissue: Umbilical cord
Morphology: Cobblestone appearance with large dark nuclei

CC₅₀/IC₅₀/EC₅₀: >0.5 mM
Exposure: 48 h
Assay: MTT
(3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide)
The MTT assay is a colorimetric assay used to assess cell viability and proliferation by measuring the reduction of MTT by mitochondrial enzymes in viable cells, resulting in the formation of purple formazan crystals.

HUVEC
(HUV-EC-C; Primary Umbilical Vein Endothelial Cells)
ATCC: pcs-100-010
 
Species: Homo sapiens (human)
Cell type: Endothelial cell
Tissue: Umbilical cord
Morphology: Cobblestone appearance with large dark nuclei

CC₅₀/IC₅₀/EC₅₀: >4.4 mM
Exposure: unknown
Assay: WST-1
Water-Soluble Tetrazolium
The WST-1 assay is a colorimetric assay used to assess cell viability and proliferation by employing a tetrazolium salt that is cleaved by cellular enzymes to produce a soluble formazan dye, which is quantified spectrophotometrically.

IPC-81
(LT12; LT-12)
Cellosaurus: CVCL_D271
 
Species: Rattus norvegicus (rat)
Disease: Acute myelocytic leukemia
Tissue: Bone marrow
 
Medium: RPMI 1640 (Gibco)

CC₅₀/IC₅₀/EC₅₀: 2.18 mM
Dispersion: 1.27 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HeLa
(He-La; HeLa-CCL2; Henrietta Lacks cells; Helacyton gartleri)
ATCC: crm-ccl-2
Cellosaurus: CVCL_0030
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Cervical adenocarcinoma
Tissue: Cervix, Uterus
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.88 mM
Dispersion: 0.55 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

HCT-116
(HCT 116; HCT.116; HCT116wt; CoCL2)
ATCC: ccl-247
Cellosaurus: CVCL_0291
 
Species: Homo sapiens (human)
Disease: Colorectal carcinoma
Tissue: Colon, Large intestine
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 1.54 mM
Dispersion: 0.48 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.

MCF-7
(Michigan Cancer Foundation-7; ssMCF7; MCF7/WT; MCF7-CTRL; IBMF-7; HTB22)
ATCC: htb-22
Cellosaurus: CVCL_0031
 
Species: Homo sapiens (human)
Cell type: Epithelial cell
Disease: Adenocarcinoma
Tissue: Breast, Mammary gland
Morphology: Epithelial
 
Medium: DMEM

CC₅₀/IC₅₀/EC₅₀: 0.1 mM
Dispersion: 0.01 (SEM)
Exposure: 24 h
Assay: MTS
(3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium)
The MTS assay is a colorimetric assay similar to the MTT assay, where a water-soluble tetrazolium salt is reduced by metabolically active cells to form a soluble formazan dye, providing a measure of cell viability and proliferation.